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SPECIES: mouse, BALB/c
DSMZ catalog code: ACC 25
Morphology: fibroblast-like cells growing as monolayers
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Description: mouse fibrosarcoma established from a fibrosarcoma chemically induced in a BALB/c mouse; cells were described to be sensitive to human cytotoxic monocytes, to tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, University of Munich, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures at 1:3 to 1:6; cells are adherent, slapping of the back of the flask by hand will detach the cells; seed out at ca. 4 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 ca. 15 x 106 cells/80 cm 2;
doubling time of ca. 30-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule;
negative in DAPI, microbiological culture assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1751
- Blood 1985;65:8-14 - PMID: 3155490
- J Natl Cancer Inst 1984;72:23-29 - PMID: 6582301
- Proc Soc Exp Biol Med 1973;144:813-818 - PMID: 4543620
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.