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ICLC catalog code: HTL97024
Continuous culture, grown as monolayer
epithelial-like, spindle and giant cells morphology
SPECIES: human (male) Karyology: 66-68 aneuploidic, pentasomy #7,multiple trisomy
TUMOR: sarcoma, epitheloid
tumorigenic in nude mice
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: GM-CSF production; receptors for GM-CSF, AE, AE3, Fas, EMA, vimentin, P53.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. Passage: 28, mycoplasma negative, HOECHST and PCR
The viability of the cells can be very low after thawing; thaw with medium supplemented with 20% FBS
Validated by: isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: ECACC: 94092303, DSMZ: ACC 328
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.