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TUMOR: lymphoma, histiocytic
DSMZ catalog code: ACC 5
Morphology: round to polygonal, single cells in suspension
Susceptibility to viruses: HIV-1 * HIV-2
Description: human histiocytic lymphoma established from the pleural effusion of a 37-year-old man with generalized diffuse histiocytic lymphoma in 1974; cells were described to express markers and properties of monocytes; patented cell line; cells express mRNA for c-myc confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Nilsson, University Hospital, Uppsala, Sweden
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 saturation density at about 2.0 x 106 cells/ml;
doubling time of about 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x10 6 cells/ampoule;
negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypotriploid (flat-moded) karyotype; 63(58-69)<3n>XXY, -2, -5, -6, +7, -9, -10, -11, -12, -12, -20, -21, +3mar, der(1)t(1;12)(p33;q13), der(5)t(1;5)(p11;q31), der(9)t(9;?)(p22;?), del(11)(q22), i(11q), der(16)t(16;?)(q24;?),) * cytogenetics 2 (der(19)t(?10;19)(q12;p13.3); expression of monocytic features may be linked to del(11q); 5q33 breakpoint near to that recurrent in histiocytic lymphoma) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for HTLV-I/II, HIV-1)
Availability in cell line catalogues: ATCC: CRL 1593, ICLC: HTL94002
- Int J Cancer 1976;17:565-577 - PMID: 178611
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1980;125:463-465 - PMID: 7381208
- Nature 1979;279:328-331 - PMID: 450085
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.