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TISSUE/ORGAN: lymphoblastoid B cell
TUMOR: leukemia, chronic myeloid
DSMZ catalog code: ACC 95
Morphology: polygonal cells, single or in clusters in suspension
Description: human B-lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis) established from the peripheral blood of a 62-year-old man with Philadelphia-chromosome negative chronic myeloid leukemia (CML) in blast crisis in 1985; cells were described to be Ph-negative, bcr-abl-negative; cells appear to be rather a 'normal' B-lymphoblastoid cell line (B-LCL) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. E. B. Thompson, University of Texas, Galveston, Texas, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Iscove's MDM + 10% FBS cells are difficult to culture! maintain at 0.2-0.8 x 106 cells/ml; split 1:1.5 to 1:2 every 2 to 3 days at 37C with 5% CO2 saturation density at about 2.5-4.0 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13- CD14- CD15- CD19+ CD20+ CD33- CD37+ HLA-DR+ anti-EBNA2+ cyIgG-cyIgM- sm_+ sm_-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
- Blood 1987;70:1665-1672 - PMID: 3478106
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.