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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
DSMZ catalog code: ACC 16
Morphology: round, single cells in suspension, partly in clusters
Susceptibility to viruses: HIV-1
Description: human acute monocytic leukemia established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978; the cells can be used for induction of differentiation studies; the cells were described to produce lysozyme and to be phagocytic confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 saturation density at about 1.0 x 106 cells/ml;
doubling time of ca. 35-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ml;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (near-tetraploid karyotype; 94(88-96)<4n>XY/XXY, -Y, +1, +3, +6, +6, -8, -13, -19, -22, -22, +2mar, add(1)(p11), del(1)(q42.2), i(2q), del(6)(p21)x2-4, i(7p), der(9)t(9;11)(p22;q23)i(9)(p10)x2, der(11)t(9;11)(p22;q23)x2, add(12)(q24) * cytogenetics 2 (der(13)t(8;13)(p11;p12), add(?18)(q21); carries t(9;11) associated with AMl M5) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: TIB 202, ICLC: HTL97014
- Cancer Res 1982;42:1530 - PMID: 6949641
- Int J Cancer 1980;26:171-176 - PMID: 6970727
- J Immunol 1983;131:1882 - PMID: 6619543
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.