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SPECIES: turtle, Terrapene carolina
ECACC catalog code: 90102528
Susceptibility to viruses: amphibian viruses * canine distemper virus * herpes simplex * pseudorabies * reptilian viruses * vaccinia * vesicular stomatitis
Description: Turtle heart
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Virus studies: HSV, VSV, PRV, CDV, Vaccinia, also amphibian and reptile viruses and reptile viruses.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2. Cells are retarded by alkaline pH. Adjust to near neutral after seeding.
Karyotype: 2n = 50
Derived from the trypsinised heart of a box turtle. Cells can develop CPE with a wide range of viruses see reference. NB. Propagation at room temperature (23) although sub lines have been adapted to grow at 30C and 36C see reference.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 50
- Exp Cell Res 1967;48:263 - PMID: 4295486
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.