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ICLC catalog code: HTL97021
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (female, 6 years old)
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: nicotinic acetylcholine receptor; acetylcholine receptor; peripheral type benzodiazepine receptor.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Seed at 2-4 x 104 cells/cm2; split confluent cultures 1:3-1:6 using trypsin/EDTA. 37C, 5% CO2. Passage: 10, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, G6PD)
Availability in cell line catalogues: ECACC: 94052610
- Brain Res 1982;231:365-367 - PMID: 7055685
- Int J Cancer 1977;20:206-212 - PMID: 892931
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.