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ICLC catalog code: HTL96023
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (female, 60 years old)
TUMOR: adenocarcinoma, grade III, poorly differentiated
tumorigenic in immunotolerant rats
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: HLA phenotype Aw24,Aw32,B27,Bw41
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5x104 cells/cm2.37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD)
Availability in cell line catalogues: IZSBS: BS TCL81
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.