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DSMZ catalog code: ACC 127
Morphology: adherent, epithelial-like cells growing in monolayers
Description: human melanoma established from the tumor biopsy of an intracranial secondary melanoma in a 28-year-old European man in 1979; it was described that increasing the tyrosine concentration enhances synthesis of melanin confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Harris & Dr. D. McCormick, Dunn School of Pathology, University of Oxford, Oxford, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 (or Leibovitz's L-15) + 10% FBS split confluent culture up to 1:10 one to two times per week using trypsin/EDTA; seed out 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 about 15 x 106 cells/175 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Br J Exp Pathol 1983;64:103-115 - PMID: 6838761
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.