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SPECIES: human, Caucasian
TUMOR: malignant melanoma
DSMZ catalog code: ACC 66
Morphology: adherent, epithelial-like cells growing as monolayers
Description: human malignant melanoma established from an involved lymph node of an 18-year-old Caucasian woman with malignant melanoma in 1971 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:4 to 1:10 every 4-6 days using trypsin/EDTA at 37C with 5% CO2 about 10-12 x 106 cells/80 cm 2;
doubling time of about 60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1.0-1.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded hyperdiploid karyotype with 22% polyploidy; 54(50-60)<2n>X, -X, +1, +2, +6, +7, +8, +20, +20, +3 mar, der(8)t(7;8)(p11;p12), der(9)t(9;11)(p21;q11), add(18)(q23;HSR); shares t(9;11) with IPC-298 defining a new recurren) * cytogenetics 2 (translocation in malignant melanoma) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 66
- J Natl Cancer Inst 1977;59:301-307 - PMID: 833878
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.