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ECACC catalog code: 94050409
Description: Rat embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
RAT-2 was derived from a subclone of a 5'-bromo-deoxyuridine (BUdR)-resistant strain of the Fischer rat 3T3-like cell line RAT-1. RAT-2 lacks appreciable levels of nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confrimation)
Availability in cell line catalogues: ATCC: CRL 1764
- Virology 1981;113:408-411 - PMID: 7269249
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.