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ICLC catalog code: HTL97018
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male, 62 years old) Karyology: triploid, modal number 62
TUMOR: adenocarcinoma, grade IV
tumorigenic in nude mice
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: HLA: A1; A9
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. Initially after thawing the viability of the cells may be low, the cells grow slowly and loosely adherent. Passage: 29, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: ECACC: 90112714, DSMZ: ACC 465
- Cancer Res 1980;40:524 - PMID: 7471073
- Invest Urol 1979;17:16 - PMID: 447482
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.