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TISSUE/ORGAN: thyroid, follicular
ECACC catalog code: 90011609
Transfected by: Transfection Back To Transfection WebPage
Description: Human thyroid follicular epithelial
Viruses: See Properties
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transfection and virus studies; study of control and growth in human thyroid
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from normal thyroid tissue of an adult. The cells have been transfected with a plasmid encoding for the SV40 large T gene.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Br J Cancer 1989;60:897 - PMID: 2557880
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.