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SPECIES: mouse, A/Jax
DSMZ catalog code: ACC 94
Morphology: adherent cells growing in monolayers or small clumps, round cell body with cellular processes
Description: mouse neuroblastoma established from a A/Jax mouse strain with neuroblastoma C1300; cells were described to be aneuploid and to contain high specific activity of choline acetyltransferase confirmed as mouse with IEF of MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. B. Hamprecht, University of Tuebingen, Tuebingen, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split semi-confluent culture 1:5 to 1:10 using trypsin/EDTA (cells can also be detached by tapping the flask); seed out at ca. 2 x 106 cells/80 cm 2 ; maximal density at ca. 5-10 x 106 cells/80 cm 2 at 37 C with 5-10% CO2 cell harvest of about 1 x 107 cells/80 cm 2;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of MDH, NP, PEP B)
- Proc Natl Acad Sci U S A 1972;69:258-263 - PMID: 4400294
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.