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SPECIES: mouse / rat
TUMOR: neuroblastoma x root ganglion neuron hybrid
ECACC catalog code: 92090912
Description: Mouse neuroblastoma x Rat neuron hybrid
Viruses: See Properties
Depositor: Dr J N Wood, Neuroimmunology Dept, The Sandoz Institute, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37C. Cells are lightly attached therefore use of trypsin is unnecessary.
Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion. The cell line has been mycoplasma eradicated at ECACC.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Proc R Soc B 1990;241:187
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.