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SPECIES: human, Caucasian
TUMOR: carcinoma, non small cell
ECACC catalog code: 94060303
Description: Human lung non-small cell carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Synthesize the peptide neuromedin B (NMB) (0.1 pmol/mg protein). Applications: Synthesis of NMB.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
A non-small cell carcinoma of the lung from a 65 year old Caucasian female taken, prior to treatment. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are not able to synthesise gastrin releasing peptide (GRP). NCI-H727 is the best differentiated available bronchial carcinoid cell line. Any proposed commercial use of these cells or products must first be negotiated with the University of Texas. More details available upon request.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 5815, ICLC: HTL97001
- Cancer Res 1992;52 (Suppl):2732-2736
- Science 1989;246:491-494 - PMID: 2554494
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.