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SPECIES: human, Caucasian
TUMOR: carcinoma, bronchioalveolar
ECACC catalog code: 95111733
Description: Human Caucasian bronchioalveolar carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
Restricted. Patent: None Specified By Depositor
Properties: Applications: Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 5-10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
NCI-H358 was isolated from a primary bronchioalveolar carcinoma of the lung from a Caucasian male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma of the lung (NSCLC) demonstrated the presence of granules characteristic of Clara cells. NCI-H358 do not express UDP-glucuronosyltransferases, but do express glutathione-S-transferase and phenol sulphotransferase. Expression of SP-A protein and RNA, the major surfactant-associated protein was detected. SP-B and SP-C RNA was not expressed. A complete homozygous deletion of the p53 gene and therefore a lack of p53 protein has been reported. A colony forming efficiency of 0.83% in soft agarose, and growth in serum-free media has been reported. The cells are tumourigenic in athymic nude mice, and exhibit a doubling time of 38 hours in RPMI 1640 medium. Since these cells are proficient in oxidation of xenobiotics but deficient in their conjugation with glucuronic acid they present a tool for analysing the role of glucuronic acid conjugation in the inactivation of chemicals in intact cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 5807
- Biochem Pharmacol 1986;35:1337 - PMID: 3083823
- Cancer Res 1986;46:798-806 - PMID: 3940644
- Cancer Res 1990;50:5481 - PMID: 2386953
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.