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TISSUE/ORGAN: bone marrow
TUMOR: leukemia,acute promyelocytic
DSMZ catalog code: ACC 207
Morphology: single cells in suspension, round and polymorphic cells
Description: human acute promyelocytic leukemia established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; patented cell line; cells carry the t(15;17) PML-RARA fusion gene confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. Lanotte, Centre Hayem, Hopital St. Louis, Paris, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio at 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5-1 x 106 cells/ml; viability may be low after thawing, but cells will recover soon when cultured initially with 20% FBS; cells might also grow better in 24-well-plates than in culture flasks at 37C with 5% CO2 cell harvest of 1.5-2.0 x 106 cells/ml;
doubling time of ca. 40-48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hypertriploid karyotype with 3% polyploidy; 78(71-81)<3n>XX,-X, +2, +6, +7, +7, +11, +12, +13, +14, +17, -19, +20, +4mar, der(8)t(8;?)(q24;?), der(11)t(11;?)(?->::11p15->11q22.1::11q13->22.1:), der(12)t(12;?)(p11;?), 14p+, (15;17)) * cytogenetics 2 ((q22;q11-12.1), der(19)t(10;19)(q21.1;p13.3)x2) * immunological analysis (CD2-, CD3-, CD4+ (60%), CD5-, CD6-, CD7-, CD8-, CD11b-, CD13+ (100%), CD14-, CD15+ (99%), CD19-, CD33+ (92%), CD38+ (92%), CD68+ (100%), TCR alfa_/á-, TCR gamma/delta_-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Blood 1991;77:1080-1086 - PMID: 1995093
- Leukemia 1992;6:1281-1287 - PMID: 1453773
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.