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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, pre-B cell
DSMZ catalog code: ACC 128
Morphology: single, small, round cells in suspension
Description: human B cell precursor leukemia established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1976 confirmed as human with IEF of AST, G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. A. Ganser, Johann-Wolfgang-Goethe University, Frankfurt, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 1.0-2.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 3 days; seed out at ca. 1 x 106 cells/ml; minimum density of 0.5 x 106 cells/ml; at 37C with 5% CO2 maximal density of 4-6 x 106 cells/ml;
doubling time of ca. 36 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x10 6 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid; 46(43-47)<2n>XY, t(5;12)(q33.2;p13.2)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37- HLA-DR+ cyIgM+ sIgM-) * isoenzymes (confirmed as human with IEF of AST, G6PDH, MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
- Int J Cancer 1979;23:174-180 - PMID: 83966
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.