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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
DSMZ catalog code: ACC 124
Morphology: single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells
Description: human acute monocytic leukemia established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia ; more information on this cell line on the following website: www.med.uni-muenchen.de/immuno/ziegler; sister cell line of MONO-MAC-1 (DSM ACC 252) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + 2 mM L-glutamine + non-essential amino acids + 1 mM sodium pyruvate + 9 ęg/ml bovine insulin maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:3 to 1:5; avoid lipopolysaccharide contamination which will result in cell clumping, maturation and retarded growth; cells are best maintained in 24-well plates; seed out at ca. 0.3 x 106 cells/ml at 37C with 5% CO2 maximal density at ca. 1.0 x 106 cells/ml;
doubling time of ca. 60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (flat-moded hypotetraploid karyotype with near-diploid (8%) and polyploid (17%) sidelines; 84-90<4n>XX/XXX, -Y, +6, +7, -12, -13, -13, -16, -16, +2mar, t(9;11)(p22;q23)x2, add(10)(p11)x2, add(12)(q21), del(13)(q13q14)de) * cytogenetics 2 (q12)x2, der(17)t(13;17)(q21;p11)x2; first cell line shown to carry t(9;11) characteristic of AML M5) * immunological analysis (CD3- CD13+ CD14+ CD15+ CD19- CD33+ HLA-DR+) * isoenzymes (confirmed as human with IEF of AST, MDH. NP) * reverse transcriptase (reverse transcriptase positive; negative for HTLV-I/II, HIV-1)
Availability in cell line catalogues: ICLC: HTL01015
- Int J Cancer 1988;41:456-461 - PMID: 3162233
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.