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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myelomonocytic
DSMZ catalog code: ACC 15
Morphology: round, single cells in suspension
Description: human acute myelomonocytic leukemia established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; sister cell lines are ML-1 and ML-3 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5 x10 6 cells/ml at 37C with 5% CO2 maximum density at 1.0-1.25 x 106 cells/ml;
doubling time of ca. 60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near tetraploid karyotype; 92(84-94)<4n>XX,-Y -Y, -7, -9, -10, -10, +11, +12, +12, +13, +13, -15, -16, -17, -17, +18, +18, -20, -20, +4mar, der(1)t(1;?)(p21;?)x2, del(6)(q23)x2, der(6)t(6;11)(q27;?q23)x2, ?der(11)t(6;11) (q27;?q2 )/del) * cytogenetics 2 ((q23)x2, der(11)t(11;?)(?->11p11->11q23:)x2, der(11)t(11;?)(q11-13;?), dup(13)(q32->qter)x2, der(18)t (15;?;18)(q21;?;q11)x2; extensive ch11 rearrangements (inc. 11q23, typical of M4 and M5 AML) obscure the cytogenetic picture;) * cytogenetics 3 (ML-2 may carry rare t(6;11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for HTLV-I/II, HIV-1)
- Cancer Genet Cytogenet 1989;37:193-200 - PMID: 2702618
- Cancer Res 1980;46:1383-1388
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.