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DSMZ catalog code: ACC 157
Morphology: adherent, fibroblast-like cells growing as monolayers
Description: human neuroblastoma established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of N-myc confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. T. Pietsch, University of Bonn, Institute of Neuropathology, Bonn, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + 2 mM L-glutamine + MEM non-essential amino acids split confluent culture 1:2 every 4 days using trypsin/EDTA; NB: medium must be changed frequently to prevent acidity which induces cell clumping; optimal dissociation after trypsination is affected by gentle aspiration with a 21 gauge needle and syringe at 37C with 5% CO2 cell harvest of about 10 x 106 cells/80 cm 2;
doubling time of ca. 100 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human bimodal hyper, -diploid/-tetraploid karyotype; 54(48-54)<2n>XY, +1, +1, +2, +6, +7, +8, +13, +20, der(1)t(1;?)(p32;?)x2, der(13)t(13;2HSR)(q33;?p23); both the long HSR on ch13 due to N-myc amplification and the ch1 short-ar) * cytogenetics 2 (rearrangements are typical of neuroblastoma) * immunological analysis (cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Anticancer Res 1988;8:1329-1334 - PMID: 3218965
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.