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DSMZ catalog code: ACC 65
Morphology: epithelial-like, loosely attached monolayers
Description: human breast carcinoma established from an effusion of a 48-year-old woman with breast carcinoma in 1976 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Leibovitz's L-15 medium + 10% FBS cells are difficult to culture! split confluent culture at 1:2 to 1:6 using trypsin/EDTA; seed out at ca. 4 x 106 cells/80 cm 2 at 37C, no exposure to CO2 (keep lid of flask closed) about 25-35 x 106 cells/80 cm 2;
doubling time of ca. 51 hours;
frozen with 85% medium, 10% FBS, 5% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid karyotype with 6% polyploidy and near diploid sideline (2%); 89(85-91)<4n>XXX, -X; highly rearranged leading to extensive marker formation, including der(5)t(5;?14)(p11;q11)x2; dm present in 12% cells) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament-vimentin-) * isoenzymes (confirmed as human with IEF of MDH, NP)
Availability in cell line catalogues: ATCC: HTB 131, ICLC: HTL01013
- Cancer Res 1979;39:919-922 - PMID: 427779
- In Vitro 1978;14:911-915 - PMID: 730202
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.