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ICLC catalog code: HTL95019
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (female, 69 years old)
Original line: MCF7
Transfected by: ER-pBact cDNA. Back To Transfection WebPage
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: variant estrogen receptor transgene
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, NP)
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.