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TUMOR: lymphoma, B cell
DSMZ catalog code: ACC 82
Morphology: round to elongated, lymphoblastoid, single or clustered cells in suspension
Description: human B cell lymphoma established from the pleural effusion of a patient with B cell lymphoma (undifferentiated lymphoma); cells were described to be EBV-negative, to carry the 8;14 translocation and to express mRNA of the proto-oncogene bcl-2 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (CRL 1649), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% RPMI 1640 + 15-20% FBS maintain at 1-2 x 106 cells/ml; split ratio of 1:2 to 1:3; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximal density at 2.5 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near diploid with 1.5% polyploidy; 46(37-46)<2n>XY, dup(1)(q21q32), t(8;14)(q24;q32), del(10)(q23)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13+ CD19+ CD37+ cy/smIgM+ cy/smIgG-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (not detected (RDDP assay))
Availability in cell line catalogues: ATCC: CRL 1649
- J Natl Cancer Inst 1980;64:465-476 - PMID: 6243721
- J Natl Cancer Inst 1980;64:477-483 - PMID: 6243722
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.