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SPECIES: Mamestra brassicae (cabbage moth)
DSMZ catalog code: ACC 85
Morphology: polymorphic round cells, partly adherent
Description: insect - cabbage moth (Mamestra brassicae) established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (Kohleule)
Depositor: Dr. H. U. Leistner, Institut fuer Pathogendiagnostik, Aschersleben, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% Grace's Insect Medium (Modified) + 15% FBS split confluent cultures 1:2 about twice a week, but without using trypsin (cells grow partly adherent, but can be detached without trypsin); maintain at room temperature (about 20C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4C for up to one week without loss of viability at 20C without CO2 (at room temperature, keep lid of flask closed) about 5-7 x 106 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
- Arch Phytopathol Pflantzenschutz 1989;25:387-401
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.