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DSMZ catalog code: ACC 265
Morphology: adherent epithelial-like cells growing as monolayer
Description: human lung adenocarcinoma established from the lung adenocarcinoma of a 63-year-old man confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Ham's F10 + 10% FBS split confluent culture 1:4 every 3 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 15 x 106 cells/175 cm 2;
doubling time of about 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded near triploid karyotype with 4% polyploidy; 69(62-76)<3n>XY, -X/Y, -1, +6, +9, -13, -14, -18, -19, +22, +3mar, add(1)(p21), i(1q), der(2)t(1;2)(p21;p23)x2, add(4)(p11/15), i(6p), del(12)(q14), i(13q), add(14)(p11),) * cytogenetics 2 (add(15)(p11), del(17)(p11)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
- J Cancer Res Clin Oncol 1989;115:S50
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.