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SPECIES: human, Caucasian
TUMOR: carcinoma, squamous cell
ECACC catalog code: 92012463
Description: Human Caucasian lung squamous cell carcinoma
Viruses: See Properties
Depositor: Dr P Rabbitts, MRC, Clinical Oncology and Radiotherapeutics Unit, Cambridge, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Control cell line for heterozygosity studies using the AGLCL cell line
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 5x1,000-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C.
Derived from a lung squamous cell carcinoma of a 72 year old Caucasian male. From the same patient as the B-lymphoblastoid cell line AGLCL (89120566).Cells grow as large swollen aggregates, which will detach and eventually grow in suspension.
Research council deposit: Yes;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Genes Chromosomes Cancer 1989;1:95
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.