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TISSUE/ORGAN: peripheral blood
TUMOR: multiple myeloma
DSMZ catalog code: ACC 41
Morphology: single, elongated, snake-like cells in suspension
Description: human multiple myeloma established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG, EBV-negative) in leukemic transformation (refractory, terminal) n 1986; cells express mRNA of proto-oncogene bcl-2 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. L. Pegoraro, University of Turin, Turin, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% Iscove's MDM + 10-20% FBS maintain at 0.5-1.0 x 106 cells/ml, cells grow slowly, split 1:2 every 2-3 days; viability may be low initially after thawing; it is recommended to cultivate the cells in 24-well-plates during the first week and to exchange medium without dilution; seed out at ca. 0.7 x 106 cells/well at 37C with 5% CO2 maximal density at about 1.0-1.5 x 106 cells/ml;
doubling time of 35-80 hours (depending on cell density);
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypertriploid karyotype; 79(75-79)<3n>XX, -X, complex karyotype including LP-1 markers: dup(1)(p13;p23), der(5)t(5;?)(q31;?), der(12)t(12;13)(p13;q11); in 33% cells 1, 2 or occasionally 4 supernumerary dm present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD19- CD37- HLA-DR+ sIgG+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
- Blood 1989;73:1020-1027 - PMID: 2784066
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.