This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
DSMZ catalog code: ACC 256
Morphology: adherent fibroblastoid cells growing in aggregates and as single cells
Description: human prostate carcinoma established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described to be androgen-sensitive confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. M. Motta, Universita di Milano, Milan, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% RPMI 1640 + 10-15% FBS split semi-confluent culture 1:4 once a week using trypsin/EDTA (occasionally cells can be detached by tapping); seed out at ca. 1-2 x 106 cells/25 cm 2 ; after thawing and trypsinization cells may need 1-2 days to become adherent again; due to the strong cell aggregation, it might be difficult to determine an exact cell titer at 37C with 5% CO2 cell harvest of about 10-15 x 106 cells/80 cm 2 flask;
doubling time of about 60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (apparently human karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%), desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Cancer Genet Cytogenet 1984;11:399-404 - PMID: 6584201
- Cancer Res 1993;43:1809-1818
- Models for Prostate Cancer (G P Murphy ed) 1981;115-132
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.