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SPECIES: mouse, C3H/An
ECACC catalog code: 90083001
Description: Mouse C34/An connective tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Gene transfer, genetic, biochemical and hybridization studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No. 45; hyperdiploid
Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. It is potentially used in genetic and biochemical studies including somatic cell hybridisation and gene transfer.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 1.3
- Exp Cell Res 1962;26:404
- Exp Cell Res 1963;31:297-312
- Proc Natl Acad Sci U S A 1972;69:510-514 - PMID: 4110482
- Proc Natl Acad Sci U S A 1980;77:1583 - PMID: 6929511
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.