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TISSUE/ORGAN: peripheral blood
TUMOR: erythremia acute
DSMZ catalog code: ACC 37
Morphology: single, large, round to oval cells in suspension
Description: human acute erythremia established from the peripheral blood of a 2-year-old girl with acute erythremia (Di Gugliemo's disease, erythroblastosis) at diagnosis in 1978; cells were described to produce hemoglobin confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. A. Kulozik, University of Ulm Medical School, Ulm, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-0.3 x 106 cells/ml at 37C with 5% CO2 maximal density of 0.5 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypotetraploid with 6% polyploidy; 82(80-88)<4n>XX, -X, -X, -10, -13, -14, -16, -18, -20, -22, +3m, der(1)t(1;?)(q21;?)x2, del(7)(p15)x2, del(9)(p12), i(17q); karyotype appears to represent derivate of that originally reported) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD19- glycophorin A-) * isoenzymes (confirmed as human with IEF of AST, MDH)
- J Cancer Res Clin Oncol 1981;102:49-55 - PMID: 6949905
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.