This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
SPECIES: human, Black
DSMZ catalog code: ACC 149
Morphology: epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present
Description: human cervix carcinoma (derivative of HELA) KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 (DSM ACC 158) which itself is a derivative of the 1951 established HELA (DSM ACC 57) (31-year-old black woman); cells express strongly mdr1 mRNA and protein confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. Gottesman, National Institutes of Health, Bethesda, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% Dulbecco's MEM + 15% FBS + 200-1000 ng/ml vinblastine cells are difficult to culture in the presence of vinblastine! split ratio of confluent cultures 1:3 to 1:10; split every 2-4 days, dependent on the vinblastine concentration, using trypsin/EDTA; upon thawing, first grow the cells without vinblastine, then in increasing amounts; seed out at ca. 3 x 106 cells/80 cm 2 in 10 ml medium; upon thawing, first grow cells without vinblastine, then in increasing amounts at 37C with 5-10% CO2 cell harvest of about 6-10 x 106 /80 cm 2;
doubling time of ca. 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid, approximately 22% cells carry variable numbers of dms; 74(71-76)<3n>) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as HELA) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
- J Biol Chem 1986;261:7762-7770 - PMID: 3711108
- Proc Natl Acad Sci U S A 1986;83:4538-4564 - PMID: 3459187
- Somat Cell Genet 1985;11:117-126 - PMID: 3856953
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.