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ECACC catalog code: 92030501
Description: Human thyroid carcinoma
Viruses: See Properties
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Wild-type p53 tumour suppressor gene Applications: Tumourigenicity studies, particularly the role of p53
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM: Ham's F12:MCDB 104 (2:1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2x1,000,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Derived from a primary papillary thyroid carcinoma. Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Mol Carcinog 1993;7:83-88
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.