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SPECIES: mouse, BALB/c
TUMOR: not defined tumor
DSMZ catalog code: ACC 170
Morphology: relatively small cells growing adherently; adherent cells will round up after a few days in culture
Description: mouse monocytes-macrophages established from a tumor in a female BALB/c mouse in 1968; cells were described to synthesize lysozyme and interleukin-1 and to have receptors for immunoglobulin and complement confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:3 to 1:4 about twice a week (prior to reaching confluence); cells detach spontaneously if allowed to exceed confluence; cells may be detached with a rubber policeman or using TNE (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl) (not trypsin/EDTA) for quantitative harvesting or for seeding out of cells collected from the supernatant for passaging; use always new culture flasks, otherwise cells are difficult to detach; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 4.5 x 107 cells/80 cm 2;
doubling time of ca. 35 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (murine hypotetraploid karyotype; 78(70-81)<4n>; in most cells a centric fragment was present) * isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B)
Availability in cell line catalogues: ATCC: TIB 67
- Cancer Res 1977;37:546-550 - PMID: 318922
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1975;114:898-905 - PMID: 1089721
- J Immunol 1977;119:950-954 - PMID: 894031
- Nature 1975;257:393-394 - PMID: 1101071
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.