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ICLC catalog code: HTL96021
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
tumorigenic in nude mice
DEPOSITOR: Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
The viability can be low after thawing; thaw with medium supplemented with 20% FBS.
Validated by: isoenzymes (confirmed as human with AST, MD)
Availability in cell line catalogues: DSMZ: ACC 165
- Cancer Res 1970;30:2110 - PMID: 5459762
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.