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ICLC catalog code: HTL95006
Continuous culture, grown as monolayer
SPECIES: human (male, 60 year old) Karyology: modal number 77
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA and CA19-9 production
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
incubate with trypsin/EDTA at 37C for at least 10-15 min. After thawing the viability of the cells may be low.
Validated by: isoenzymes (confirmed as human with MD, MPI, NP)
Availability in cell line catalogues: ECACC: 93121056, DSMZ: ACC 223
- Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.