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ICLC catalog code: HTL95010
Continuous culture, grown as suspension
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10; maintain cultures between 5-9x105 cells/ml; 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
After treatment with DMSO the cells differentiate along the granulocytic line (after 4 days in 1,25% DMSO, 72% of the cells become CD11b positive).
Validated by: isoenzymes (confirmed as human with MD, MPI, NP)
Availability in cell line catalogues: DSMZ: ACC 3
- Blood 1979;54:713-733 - PMID: 288488
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci USA 1978;75:2458-2462 - PMID: 276884
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.