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SPECIES: human, Caucasian
ECACC catalog code: 86030501
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * herpes simplex * measles * Newcastle disease virus * poliovirus 1 * respiratory syncytial virus * vesic. stomatitis (Indiana)
Description: Human Caucasian larynx carcinoma, HeLa Characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. After thawing the 1st subculture may take 7-10 days.
Karyotype: Modal No 14
Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of 56 year old male. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 23
- Cancer Res 1954;14:660
- Cancer Res 1955;15:598
- J Clin Microbiol 1990;28:1049 - PMID: 2161864
- J Gen Virol 1983;64:825 - PMID: 6834007
- Virology 1969;38:42 - PMID: 4306525
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.