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ECACC catalog code: 93120822
Susceptibility to viruses: adenovirus 3 * adenovirus 4 * adenovirus 7 * canine distemper virus * Coxsackie A11 * Coxsackie A13 * Coxsackie A15 * Coxsackie A18 * Coxsackie B * echovirus * ECM * herpes * measles * mumps * Newcastle disease virus * parainfluenza 2 * poliovirus 1 * poliovirus 3 * RS * vesic. stomatitis (Indiana)
Description: Human heart, HeLa characteristics
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 45
Derived from a biopsy specimen of the right auricular appendage of an adult human heart. The cells support the growth of polio viruses 1 and 3, adenovirus 3 and VSV (Indiana strain). This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: DNA fingerprinting (HeLa profile) * isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 47, IZSBS: BS CL 47
- Fed Proc 1975;16:414
- J Natl Cancer Inst 1964;33:285
- Proc Soc Exp Biol Med 1958;98:18
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.