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ICLC catalog code: ATL96006
Continuous culture, grown as monolayer
SPECIES: rat, Wistar-Furth (female, 7 months old)
TUMOR: pituitary adenoma
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells were used for studies on growth hormon and prolactin secretion.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F10 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
After thawing the viability of the cells may be low
Validated by: isoenzymes (confirmed as rat with AST, G6PD, PepB)
- J Gen Physiol 1984;83:309-339 - PMID: 6325585
- Proc Natl Acad Sci USA 1985;82:4282-4286 - PMID: 2408278
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.