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ECACC catalog code: 95091901
Transfected by: Transfection Back To Transfection WebPage
Description: Human fibrosarcoma (retroviral packaging cells)
Viruses: See Properties
Depositor: Dr Y Takeuchi, The Institute of Cancer Research, London, UK (Originator)
Restricted. Patent: None Specified By Depositor
Properties: Applications: Packaging of retrovirus vector.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:20 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
FLYA13 has been developed together with FLYRD18 (95091902) for packaging of retrovirus, it is especially useful for in vivo gene therapy . Both cell lines have been derived from HT1080 cells (85111505). FLYA13 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of amphotropic murine leukaemia virus. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000 i.u./ml. Addition of 4µg/ml blasticidin S and 10µg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmayion)
- J Virol 1995;69:7430 - PMID: 7494248
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.