IRCCS AOU San Martino IST, Genova, Italy

European Collection for Biomedical Research

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ECBR Collection

ECBR is a non-profit institution founded in 1986 as a joint-project between the Immunogenetics Laboratory of the National Cancer Research Institute of Genova, Italy and the Institute of Immunology, Universitatsklinikum of Essen, Germany.

ECBR

ECBR cell bank is now one of the collections of the institutional Biological Resource Centre (CRB-USMI) of the Ospedale Policlinico San Martino, Genova, Italy.
The aim of ECBR is the production (through EBV-immortalization), characterization, collection, preservation and distribution of human HLA-typed cell lines including HLA homozygous B-LCLs collected from the 9th, 10th, 12th and 4th Asia-Oceania International Histocompatibility Workshops (IHW) and HLA heterozygous B-LCLs and/or PBLs from unrelated healthy individuals, after informed consent, derived from Centro Ricerche Immunoematologiche AVIS, Bergamo, Italy.
Furthermore, ECBR includes murine HLA-transfectants collected from the 11th IHW available at HyperCLDB site, an hypertextual databank of the main cell culture collections in Europe.
At present, about 500 cell lines are available in ECBR bank for distribution to the interested institutions, on a partial cost recovery basis. The cell lines are tested for mycoplasma by Hoechst staining and PCR, for typing of HLA-Class I and Class II alleles by PCR-based (SSP, SSOP) and sequence-based (SBT) techniques.
Murine transfectants are tested for HLA-expression by immunofluorescence and flow cytometry or molecular methods.