ICLC catalog code: AL00003
Continuous culture, grown as suspension
single cells and few small clusters morphology
SPECIES: mouse, C57BL/6
TISSUE/ORGAN: lymphocyte, T
DEPOSITOR: Dr S. Ferrini, Immunopharmacolgy Dept, IST at ABC, Genoa, Italy
Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
PROPERTIES: The cells are dependent on Interleukin-2 (IL-2) for growth and are used as an assay system for this factor
Available in the following LABORATORY:
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 50 u/ml IL-2 + 4.7 x 10^-5M 2-mercaptoethanol; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 3x10^4 and 1x10^5 cells/ml; split 1:5-1:20. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
After thawing the viability of the cells may be very low. Thaw the cells with medium with 20% FBS and seed in 24-well-plates at density of 2-6 x 10^5 cells/ml.
Validated by: isoenzymes (confirmed as mouse with MD, MPI)
Availability in cell line catalogues: ECACC: 93042610
- Nature 1977;268:154-156 - PMID: 145543
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.