ECACC catalog code: 85050301
Endogenous viruses: contains HIV-1
Susceptibility to viruses: HIV-1 * HLTV-III
Description: Human T cell
Viruses: See Properties
Depositor: Dr R Downing, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: CD3, CD4 Applications: Detection, isolation and propagation of HIV-1
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Modal No 69
Clonal derivative of the T-cell line HUT 78 derived from the blood of a patient with Sezary syndrome. The clone was selected due to its suitability for the isolation and continuous high level production and HIV variants with patients within AIDS and pre-AIDS. Examination with DNA probes indicate identical HLA typing and DNA fingerprints as HuT-78 (Aids Res and Hum Retroviruses 1989).
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 8543, ICLC: HTL05006
- AIDS Res Hum Retroviruses 1989;5:253 - PMID: 2567177
- Lancet 1984;2:1472 - PMID: 6151082
- Science 1984;224:497-500 - PMID: 6200935
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.