SPECIES: mouse, Swiss albino
ECACC catalog code: 86052701
Morphology: Fibroblast-like. Adipose like when confluent
Description: Mouse embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Calf Serum (CS). Split confluent cultures 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 in a 75 cm2 flask using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Never allow the culture to become fully confluent and subculture every 3 days.
Karyotype: 2n = 40; Anueploid with unstable karyotype
3T3 L1 is a continuous strain of 3T3 developed through clonal isolation. Cells undergo a pre-adipose to adipose-like conversion as they progress from rapidly dividing to a confluent and contact inhibited state. A high serum content in the culture medium reportedly enhances the rapidity and degree of fat accumulation.
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 92.1, IZSBS: BS CL 100
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.