SPECIES: mouse, C3H/He
ECACC catalog code: 95102107
Description: Mouse teratocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Alpha MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2.5% Fetal Bovine Serum (FBS) + 7.5% Calf Serum (CS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The P19 cell line was derived from an embryonal carcinoma induced in a C3H/He strain mouse. The cells can be cloned at high efficiency in medium containing 0.1mM beta-mercaptoethanol. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1825, ICLC: ATL99013
- Dev Biol 1982;89:503 - PMID: 7056443
- J Cell Biol 1982;94:253 - PMID: 7107698
- Nature 1982;299:165 - PMID: 7110336
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.