SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
ECACC catalog code: 95011815
Morphology: Epitheloid and Neuroblastoid sub-populations
Description: Human Caucasian neuroblastoma
Viruses: See Properties
Depositor: Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, NY, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chemotherapy development, chromosome studies, drug resistance, differentiation studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham's F12 (1:1) + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 44
Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HTL96015
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- J Natl Cancer Inst 1976;57:683-695 - PMID: 62055
- Life Sci 1991;50:7-11
- Science 1976;191:185-187 - PMID: 942798
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.