DSMZ catalog code: ACC 199
Morphology: adherent epithelial-like cells growing as monolayers
Description: rat kidney epithelial-like cells cell line was cloned from a mixed culture of normal rat kidney cells as was NRK-49F (DSM ACC 172); the two cell lines were described to be distinct in growth properties and in transforming abilities; NRK-52E is supposed to be much more stable (= ATCC CRL 1571) confirmed as rat with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% Dulbecco's MEM + 5-10% FBS split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 3-5 x 10 6 /80 cm 2;
doubling time of ca. 45 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 4% tetraploidy; 41/42(36-42)<2n>XX; hypodiploid sideline with 1q+ marker (18%); near triploid sideline with additional structural and numerical rearrangements (2%)) * isoenzymes (confirmed as rat with IEF of AST, MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CRL 1571, ECACC: 87012902
- J Cell Physiol 1978;94:335 - PMID: 304450
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.