ICLC catalog code: HL95002
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (14-18 weeks old)
TISSUE/ORGAN: foreskin, fetal
Susceptibility to viruses: cytomegalovirus * herpes simplex
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-3x104 cells/cm2; 37C, 5% CO2. The cells grow very slowly. Passage: 14 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with MD, MPI, NP)
Availability in cell line catalogues: ECACC: 86031405
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.